An interjection at the start of a sentence can be followed by a comma or an exclamation mark.
Yes Please Comma. Use a comma before please at the end of a request. Use a comma after yes and no.
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Even the slickest grammarian struggles with proper comma use, so we compiled a list of the places in a sentence you'll need one — using ducks. By adding the comma after please, the sentence changes tone, and the please sounds more irritated and exasperated. A polite formula for accepting an offer , invitation , etc | meaning, pronunciation, translations and times, sunday times (2008).
Whether to use a comma or not depends on what you mean to say.
Use a comma if please is at the end of a request. Commas and periods are the most frequently used punctuation marks. Commas can be a particularly tricky punctuation mark. Don't use a comma if please is at the beginning of a request.